SCFbTrCP1 complex, active
Complex of N-Terminal GST-tagged, recombinant, human βTrCP1, amino acid residues 53-end, N-Terminal GST-tagged, recombinant, human Skp1, full length, N-Terminal 6His-tagged, recombinant, human Cul1, full length and untagged recombinant, human Rbx1, full length, co-expressed by baculovirus in Sf21 insect cells.
Biological information
Background
SCFbTrCP1 complex is a type of E3 ubiquitin ligase that targets specific proteins for degradation by the proteasome through the addition of ubiquitin molecules. The addition of ubiquitin to a protein serves as a signal for the proteasome to degrade the protein. In particular, the SCFbTrCP1 complex recognizes specific protein substrates and transfers ubiquitin molecules onto lysine residues of the target protein. The number and pattern of ubiquitin molecules added to the protein can affect the fate of the protein and determine the extent and specificity of its degradation by the proteasome.
Target class
Ubiquitin
Family
E3
Accession number
GenBank NM_033637 βTrCP1
Target Name
SCFbTrCP1 complex, active
Target Alias
Origin
Human
Theori. MW
91 kDa (βTrCP1), 46 kDa (Skp1), 93 kDa(Cul1) and 12 kDa (Rbx1)
Affinity tag
N-terminal GST-tag βTrCP1 / N-Terminal GST-tag Skp1 / N-Terminal 6His-tag Cul1 / untagged Rbx1
Product specifications
Expression system
expressed in insect cells
Purity
92%
Purification method
Glutathione sepharose
Sample Buffer
1.504 mg/ml of enzyme in 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
SCFbTrCP1 complex is a type of E3 ubiquitin ligase that targets specific proteins for degradation by the proteasome through the addition of ubiquitin molecules. The addition of ubiquitin to a protein serves as a signal for the proteasome to degrade the protein. In particular, the SCFbTrCP1 complex recognizes specific protein substrates and transfers ubiquitin molecules onto lysine residues of the target protein. The number and pattern of ubiquitin molecules added to the protein can affect the fate of the protein and determine the extent and specificity of its degradation by the proteasome.
Compound name
Ubiquitin
Catalog number
23-026
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
23-026
Datasheet