b-catenin, activated
N-terminal c-Myc, 6His-tagged, recombinant human β-catenin full length, expressed by baculovirus in Sf21 insect cells.
Biological information
Background
β-catenin is a multifunctional protein; it is a central component of the cadherin cell adhesion complex, regulating the control of cell adhesion and plays an essential role in the Wingless/Wnt signaling pathway that governs cell fate in early embryogenesis. The regulation of β-catenin turnover in the cells is governed by its phosphorylation status which allows the targeting of ubiquitin-dependent proteolysis. Altered levels of protein expression as well as genetic mutations of β-catenin have been implicated in a number of human cancers. Expected Purity 86% by SDS-PAGE and Coomassie blue staining. Purified using immobilized metal affinity chromatography.
Target class
Ubiquitin
Family
Substrate
Accession number
GenBank NM_001904
Target Name
b-catenin, activated
Target Alias
B-catenin, beta-catenin, ctnnb-a, ctnnb1-b, ctnnb1
Origin
Human
Theori. MW
91 kDa
Affinity tag
N-terminal c-Myc, 6His-tag
Product specifications
Expression system
expressed in insect cells
Purity
86%
Purification method
Immobilised metal affinity chromatography
Sample Buffer
0.49 mg/ml of enzyme in 25 mM MOPS pH 7.5, 5 mM MgCl2, 0.01% Tween 20, 1 mM ATP. Frozen solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
β-catenin is a multifunctional protein; it is a central component of the cadherin cell adhesion complex, regulating the control of cell adhesion and plays an essential role in the Wingless/Wnt signaling pathway that governs cell fate in early embryogenesis. The regulation of β-catenin turnover in the cells is governed by its phosphorylation status which allows the targeting of ubiquitin-dependent proteolysis. Altered levels of protein expression as well as genetic mutations of β-catenin have been implicated in a number of human cancers. Expected Purity 86% by SDS-PAGE and Coomassie blue staining. Purified using immobilized metal affinity chromatography.
Compound name
Ubiquitin
Catalog number
23-027
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
23-027
Datasheet