IkBa, activated
N-terminal c-Myc, 6His-tagged, recombinant human IκBα full length, expressed by baculovirus in Sf21 insect cells.
Biological information
Background
Phosphorylated in vitro using IKKα, active (Item # 14-461).
IκBα is a protein that acts as an inhibitor of the transcription factor NF-κB. NF-κB is a transcription factor that plays a critical role in regulating the expression of genes involved in inflammation, immune response, and cell survival. Purified using immobilized metal affinity chromatography.
Target class
Ubiquitin
Family
Substrate
Accession number
GenBank M69043
Target Name
IkBa, activated
Target Alias
EDAID2, IKBA, MAD-3, NFKBI
Origin
Human
Theori. MW
#N/A
Affinity tag
#N/A
Product specifications
Expression system
expressed in insect cells
Purity
70%
Purification method
Immobilised metal affinity chromatography
Sample Buffer
0.22mg/ml of enzyme in 25mM MOPS pH7.5, 5mM
MgCl2, 0.01% Tween 20, 1mM ATP. Frozen solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
Phosphorylated in vitro using IKKα, active (Item # 14-461).
IκBα is a protein that acts as an inhibitor of the transcription factor NF-κB. NF-κB is a transcription factor that plays a critical role in regulating the expression of genes involved in inflammation, immune response, and cell survival. Purified using immobilized metal affinity chromatography.
Compound name
Ubiquitin
Catalog number
23-028
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
23-028
Datasheet