SCFFbw7 complex, active
Complex of N-terminal GST-tagged, recombinant human Fbw7 full length, N-terminal 6His-tagged, recombinant human Skp1 full length, N-terminal 6His-tagged, recombinant human Cul1 full length and untagged recombinant human Rbx1 full length, co-expressed by baculovirus in Sf21 insect cells.
Biological information
Background
The SCF (Skp1-Cul1-F-box protein) complexes represent the largest family of ubiquitin-protein ligases and mediate the ubiquitination of a broad spectrum of regulatory and signalling proteins in diverse cellular pathways. SCFFbw7 mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell division, growth and differentiation, including Cyclin E1, c-Myc, Notch and c-Jun. Mutations have been found in the Fbw7 subunit of SCFFbw7 in various types of human cancers, therefore Fbw7 is considered to be a potent tumour suppressor. Expected Purity 93% by SDS-PAGE and Coomassie blue staining.
Target class
Ubiquitin
Family
E3
Accession number
GenBank NM_033632
Target Name
SCFFbw7 complex, active
Target Alias
AGO, CDC4, DEDHIL, FBW6, FBW7, FBX30, FBXO30, FBXW6, SEL-10, SEL10, hAgo, hCdc4
Origin
Human
Theori. MW
107 kDa (Fbw7), 21 kDa (Skp1), 93 kDa (Cul1) and 12 kDa (Rbx1)
Affinity tag
N-terminal GST-tag Fbw7 / N-Terminal 6His-tag Skp1 / N-Terminal 6His-tag Cul1 / untagged Rbx1
Product specifications
Expression system
expressed in insect cells
Purity
93%
Purification method
Glutathione sepharose
Sample Buffer
0.41 mg/ml of enzyme in 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
The SCF (Skp1-Cul1-F-box protein) complexes represent the largest family of ubiquitin-protein ligases and mediate the ubiquitination of a broad spectrum of regulatory and signalling proteins in diverse cellular pathways. SCFFbw7 mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell division, growth and differentiation, including Cyclin E1, c-Myc, Notch and c-Jun. Mutations have been found in the Fbw7 subunit of SCFFbw7 in various types of human cancers, therefore Fbw7 is considered to be a potent tumour suppressor. Expected Purity 93% by SDS-PAGE and Coomassie blue staining.
Compound name
Ubiquitin
Catalog number
23-030
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
23-030
Datasheet