Cyclin E1 complex
Complex of N-terminal c-Myc, 6His-tagged, recombinant human Cyclin E1 full length and untagged, recombinant human CDK2 full length, co-expressed by baculovirus in Sf21 insect cells.
Biological information
Background
Cyclin E1 functions as a regulator of the cell cycle, promoting the G1 to S phase transition by binding to and activating the cyclin dependent kinase CDK2. The abundance of cyclin E1 fluctuates during a normal cell cycle and is controlled at the level of gene transcription and by ubiquitin dependent proteolysis. Non-complexed cyclin E1 is degraded by the Cul3 pathway, however when cyclin E1 is bound to CDK2 its ubiqutination is dependent on a positive phosphorylation status, which enables its recognition by SCFFbw7. The impact of cyclin E1 quantities on cell cycle regulation is reflected in the elevated levels expressed by many types of human tumours. Expected Purity 64% by SDS-PAGE and Coomassie blue staining. Purified using immobilized metal affinity chromatography.
Target class
Ubiquitin
Family
Substrate
Accession number
GenBank NM_001238
Target Name
Cyclin E1 complex
Target Alias
CCNE, pCCNE1
Origin
Human
Theori. MW
52 kDa (Cyclin E1) and 34 kDa (CDK2)
Affinity tag
N-terminal c-Myc, 6His-tag Cyclin E1 / untagged CDK2
Product specifications
Expression system
expressed in insect cells
Purity
64%
Purification method
Immobilised metal affinity chromatography
Sample Buffer
1.20 mg/ml of enzyme in 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
Cyclin E1 functions as a regulator of the cell cycle, promoting the G1 to S phase transition by binding to and activating the cyclin dependent kinase CDK2. The abundance of cyclin E1 fluctuates during a normal cell cycle and is controlled at the level of gene transcription and by ubiquitin dependent proteolysis. Non-complexed cyclin E1 is degraded by the Cul3 pathway, however when cyclin E1 is bound to CDK2 its ubiqutination is dependent on a positive phosphorylation status, which enables its recognition by SCFFbw7. The impact of cyclin E1 quantities on cell cycle regulation is reflected in the elevated levels expressed by many types of human tumours. Expected Purity 64% by SDS-PAGE and Coomassie blue staining. Purified using immobilized metal affinity chromatography.
Compound name
Ubiquitin
Catalog number
23-031
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
23-031
Datasheet