MDM2/CK1d, active
N-terminal GST-tagged, recombinant, human MDM2, full length, expressed in E. coli.
Biological information
Background
Purified using glutathione sepharose followed by gel filtration. Combined in vitro with CK1δ, active (Item # 14-520).
MDM2 (Mouse Double Minute 2) is an E3 ubiquitin ligase that targets the tumor suppressor protein p53 for degradation by the proteasome. CK1δ (Casein Kinase 1 Delta) is a protein kinase that can phosphorylate MDM2. MDM2 can also be ubiquitinated by other E3 ubiquitin ligases, such as CHIP (C-terminus of Hsc70-Interacting Protein) and RNF5 (Ring Finger Protein 5), which can lead to MDM2 degradation. However, CK1δ-mediated phosphorylation of MDM2 has been shown to prevent its degradation by CHIP and RNF5, leading to increased stability of MDM2.
Overall, the ubiquitination of MDM2 by CK1δ and other E3 ubiquitin ligases plays a crucial role in regulating the stability and activity of MDM2 and p53, which are important players in tumor suppression and cell survival.
Target class
Ubiquitin
Family
E3
Accession number
GenBank AF527840
Target Name
MDM2/CK1d, active
Target Alias
ACTFS, HDMX, LSKB, hdm2, ASPS, CKI-delta, CKId, CKIdelta, FASPS2, HCKID
Origin
Human
Theori. MW
82 kDa (MDM2) and 61 kDa (CK1δ)
Affinity tag
N-terminal GST-tag MDM2
Product specifications
Expression system
expressed in E. coli cells
Purity
75%
Purification method
Glutathione sepharose
Sample Buffer
0.120 mg/ml of enzyme in 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
Purified using glutathione sepharose followed by gel filtration. Combined in vitro with CK1δ, active (Item # 14-520).
MDM2 (Mouse Double Minute 2) is an E3 ubiquitin ligase that targets the tumor suppressor protein p53 for degradation by the proteasome. CK1δ (Casein Kinase 1 Delta) is a protein kinase that can phosphorylate MDM2. MDM2 can also be ubiquitinated by other E3 ubiquitin ligases, such as CHIP (C-terminus of Hsc70-Interacting Protein) and RNF5 (Ring Finger Protein 5), which can lead to MDM2 degradation. However, CK1δ-mediated phosphorylation of MDM2 has been shown to prevent its degradation by CHIP and RNF5, leading to increased stability of MDM2.
Overall, the ubiquitination of MDM2 by CK1δ and other E3 ubiquitin ligases plays a crucial role in regulating the stability and activity of MDM2 and p53, which are important players in tumor suppression and cell survival.
Compound name
Ubiquitin
Catalog number
23-032
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
23-032
Datasheet