MDM2 (c-Myc tagged), active
N-terminal c-Myc, GSTtagged, recombinant human MDM2 full length, expressed in E.coli.
Biological information
Background
MDM2 is an E3 ubiquitin ligase that regulates the turnover of several cellular factors including the p53 tumour suppressor protein and notably MDM2 itself. It contains a number of functional elements including an N-terminal p53 binding domain, a C-terminal RING finger domain that mediates ubiquitin transfer, and a central acidic region critical for MDM2 function. Several human tumour types have been shown to have increased levels of MDM2, including soft tissue sarcomas, osteosarcomas and breast tumours. Expected Purity 63% by SDS-PAGE and Coomassie blue staining.
Target class
Ubiquitin
Family
E3
Accession number
GenBank AF527840
Target Name
MDM2 (c-Myc tagged), active
Target Alias
HDMX, LSKB, hdm2, ASPS
Origin
Human
Theori. MW
83 kDa
Affinity tag
N-terminal c-Myc, GST-tag
Product specifications
Expression system
expressed in E. coli cells
Purity
63%
Purification method
Glutathione sepharose
Sample Buffer
0.24 mg/ml of enzyme in 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
MDM2 is an E3 ubiquitin ligase that regulates the turnover of several cellular factors including the p53 tumour suppressor protein and notably MDM2 itself. It contains a number of functional elements including an N-terminal p53 binding domain, a C-terminal RING finger domain that mediates ubiquitin transfer, and a central acidic region critical for MDM2 function. Several human tumour types have been shown to have increased levels of MDM2, including soft tissue sarcomas, osteosarcomas and breast tumours. Expected Purity 63% by SDS-PAGE and Coomassie blue staining.
Compound name
Ubiquitin
Catalog number
23-033
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
23-033
Datasheet