p53
Nterminal c-Myc, 6His-tagged, recombinant human p53 full length, expressed by baculovirus in Sf21 insect cells.
Biological information
Background
p53 is expressed in a wide variety of tissues and plays a major role in preventing tumour development. It is involved in cell cycle regulation, functioning as a trans-activator to negatively regulate cell division by controlling a set of genes required for this process. It acts as a tumour suppressor in many tumour types by stimulating either apoptosis or growth arrest depending on the physiological circumstances and the cell type. Defects in p53 expression have been implicated in several diseases including; choroid plexus papilloma, lung cancer, head/neck squamous cell carcinomas, oesophageal squamous cell carcinoma, Li-Fraumeni syndrome, and hereditary adrenocortical carcinoma. Expected Purity 90.8% by SDS-PAGE and Coomassie blue staining.
Target class
Ubiquitin
Family
Substrate
Accession number
GenBank BC003596
Target Name
p53
Target Alias
BCC7, BMFS5, LFS1, P53, TRP53
Origin
Human
Theori. MW
49 kDa
Affinity tag
N-terminal c-Myc, 6His-tag
Product specifications
Expression system
expressed in insect cells
Purity
91%
Purification method
Immobilized metal affinity chromatography
Sample Buffer
1.32 mg/ml of enzyme in 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
p53 is expressed in a wide variety of tissues and plays a major role in preventing tumour development. It is involved in cell cycle regulation, functioning as a trans-activator to negatively regulate cell division by controlling a set of genes required for this process. It acts as a tumour suppressor in many tumour types by stimulating either apoptosis or growth arrest depending on the physiological circumstances and the cell type. Defects in p53 expression have been implicated in several diseases including; choroid plexus papilloma, lung cancer, head/neck squamous cell carcinomas, oesophageal squamous cell carcinoma, Li-Fraumeni syndrome, and hereditary adrenocortical carcinoma. Expected Purity 90.8% by SDS-PAGE and Coomassie blue staining.
Compound name
Ubiquitin
Catalog number
23-034
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
23-034
Datasheet