UbcH6, active
N-Terminal HA and 6His-tagged, recombinant, human UbcH6, full length, expressed in E. coli.
Biological information
Background
UbcH6 (Ubiquitin-conjugating enzyme H6) is an E2 ubiquitin-conjugating enzyme that plays a role in the ubiquitination pathway. Specifically, UbcH6 is known to participate in the ubiquitination of various substrates, including p53, MDM2, and HIF-1α.
In the ubiquitination process, UbcH6 works in conjunction with an E1 ubiquitin-activating enzyme and an E3 ubiquitin ligase to transfer ubiquitin molecules to target proteins. UbcH6 binds to the activated ubiquitin molecule and forms a thioester bond between the C-terminal glycine residue of ubiquitin and the active site cysteine residue of UbcH6. Once the ubiquitin molecule is bound to UbcH6, it can be transferred to the target protein through an isopeptide bond formed between a lysine residue on the target protein and the C-terminal glycine residue of ubiquitin.
Target class
Ubiquitin
Family
E2
Accession number
GenBank NM_003341
Target Name
UbcH6, active
Target Alias
UBE2E1, UBCH6
Origin
Human
Theori. MW
24 kDa
Affinity tag
N-terminal HA, 6His-tag
Product specifications
Expression system
expressed in E. coli cells
Purity
96%
Purification method
Immobilized metal affinity chromatography
Sample Buffer
2.49 mg/ml of enzyme in 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
UbcH6 (Ubiquitin-conjugating enzyme H6) is an E2 ubiquitin-conjugating enzyme that plays a role in the ubiquitination pathway. Specifically, UbcH6 is known to participate in the ubiquitination of various substrates, including p53, MDM2, and HIF-1α.
In the ubiquitination process, UbcH6 works in conjunction with an E1 ubiquitin-activating enzyme and an E3 ubiquitin ligase to transfer ubiquitin molecules to target proteins. UbcH6 binds to the activated ubiquitin molecule and forms a thioester bond between the C-terminal glycine residue of ubiquitin and the active site cysteine residue of UbcH6. Once the ubiquitin molecule is bound to UbcH6, it can be transferred to the target protein through an isopeptide bond formed between a lysine residue on the target protein and the C-terminal glycine residue of ubiquitin.
Compound name
Ubiquitin
Catalog number
23-036
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
23-036
Datasheet