Smad7
N-Terminal c-Myc and 6His-tagged, recombinant, human Smad7, full length, expressed by baculovirus in Sf21 insect cells.
Biological information
Background
Smad7 plays a key role in the TGF-β signaling pathway. It is a member of the Smad family of proteins, which are intracellular signaling molecules that transduce signals from TGF-β receptors to the nucleus. Smad7 functions as a negative regulator of TGF-β signaling by interfering with the activation of downstream effectors, including Smad2 and Smad3. Specifically, Smad7 can inhibit the phosphorylation of Smad2/3 by the TGF-β receptor, which is required for their activation and subsequent translocation to the nucleus to regulate gene expression.
Smad7 can also act as an E3 ubiquitin ligase, promoting the degradation of TGF-β receptor complexes. Smad7 can bind to the TGF-β receptor and recruit the E2 ubiquitin-conjugating enzyme UbcH7, leading to the ubiquitination and degradation of the receptor complex by the proteasome.
Target class
Ubiquitin
Family
E3
Accession number
GenBank NM_005904
Target Name
Smad7
Target Alias
CRCS3, MADH7, MADH8
Origin
Human
Theori. MW
51 kDa
Affinity tag
N-terminal c-Myc, 6His-tag
Product specifications
Expression system
expressed in insect cells
Purity
89%
Purification method
Immobilized metal affinity chromatography
Sample Buffer
0.895 mg/ml of enzyme in 50 mM Tris/HCl pH 8.0, 150 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
Smad7 plays a key role in the TGF-β signaling pathway. It is a member of the Smad family of proteins, which are intracellular signaling molecules that transduce signals from TGF-β receptors to the nucleus. Smad7 functions as a negative regulator of TGF-β signaling by interfering with the activation of downstream effectors, including Smad2 and Smad3. Specifically, Smad7 can inhibit the phosphorylation of Smad2/3 by the TGF-β receptor, which is required for their activation and subsequent translocation to the nucleus to regulate gene expression.
Smad7 can also act as an E3 ubiquitin ligase, promoting the degradation of TGF-β receptor complexes. Smad7 can bind to the TGF-β receptor and recruit the E2 ubiquitin-conjugating enzyme UbcH7, leading to the ubiquitination and degradation of the receptor complex by the proteasome.
Compound name
Ubiquitin
Catalog number
23-039
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
23-039
Datasheet