Src
N-Terminal c-Myc and 6His- tagged, recombinant, human Src, full length, expressed by baculovirus in Sf21 insect cells.
Biological information
Background
Src plays a role in various cellular processes, including cell proliferation, differentiation, and survival. The activity of Src is tightly regulated by several mechanisms, including ubiquitination and proteasomal degradation. Ubiquitination of Src can have different effects on its activity, depending on the site of ubiquitination and the type of ubiquitin chain that is formed. The ubiquitination of Src can be regulated by various factors, including kinases, phosphatases, and E3 ubiquitin ligases. For example, the E3 ubiquitin ligase Cbl can ubiquitinate Src and promote its degradation by the proteasome, while other E3 ubiquitin ligases, such as Nedd4 and CHIP, can also regulate Src activity through ubiquitination.
Target class
Ubiquitin
Family
Substrate
Accession number
GenBank NM_198291
Target Name
Src
Target Alias
ASV1, THC6, c-SRC, p60-Src, SRC
Origin
Human
Theori. MW
65 kDa
Affinity tag
N-terminal c-Myc, 6His- tag
Product specifications
Expression system
expressed in insect cells
Purity
94%
Purification method
Immobilized metal affinity chromatography
Sample Buffer
1.965 mg/ml of enzyme in 50 mM Tris/HCl pH 7.5, 150 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
Src plays a role in various cellular processes, including cell proliferation, differentiation, and survival. The activity of Src is tightly regulated by several mechanisms, including ubiquitination and proteasomal degradation. Ubiquitination of Src can have different effects on its activity, depending on the site of ubiquitination and the type of ubiquitin chain that is formed. The ubiquitination of Src can be regulated by various factors, including kinases, phosphatases, and E3 ubiquitin ligases. For example, the E3 ubiquitin ligase Cbl can ubiquitinate Src and promote its degradation by the proteasome, while other E3 ubiquitin ligases, such as Nedd4 and CHIP, can also regulate Src activity through ubiquitination.
Compound name
Ubiquitin
Catalog number
23-042
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
23-042
Datasheet