Kit
N-Terminal c-Myc and GST-tagged, recombinant, human Kit, amino acid residues 544-end, expressed by baculovirus in Sf21 insect cells.
Biological information
Background
Recombinant human Kit, also known as recombinant human stem cell factor (SCF), is a protein that has been produced using recombinant DNA technology. The Kit protein is a transmembrane receptor tyrosine kinase that binds to SCF, which is its ligand.
Recombinant human Kit has been used in clinical applications, particularly in the context of stem cell transplantation. SCF is a critical factor for the survival and proliferation of hematopoietic stem cells, and recombinant human Kit has been used to support the expansion of these cells in vitro before transplantation. Additionally, recombinant human Kit has been used in clinical trials as a potential treatment for conditions such as acute myeloid leukemia and gastrointestinal stromal tumors, which are characterized by mutations in the Kit gene.
Target class
Ubiquitin
Family
Substrate
Accession number
GenBank X06182
Target Name
Kit
Target Alias
C-Kit, CD117, MASTC, PBT, SCFR
Origin
Human
Theori. MW
78 kDa
Affinity tag
N-terminal c-Myc, GST-tag
Product specifications
Expression system
expressed in insect cells
Purity
81%
Purification method
Glutathione sepharose
Sample Buffer
0.39 mg/ml of enzyme in 50 mM Tris/HCl pH 7.5, 150 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
Recombinant human Kit, also known as recombinant human stem cell factor (SCF), is a protein that has been produced using recombinant DNA technology. The Kit protein is a transmembrane receptor tyrosine kinase that binds to SCF, which is its ligand.
Recombinant human Kit has been used in clinical applications, particularly in the context of stem cell transplantation. SCF is a critical factor for the survival and proliferation of hematopoietic stem cells, and recombinant human Kit has been used to support the expansion of these cells in vitro before transplantation. Additionally, recombinant human Kit has been used in clinical trials as a potential treatment for conditions such as acute myeloid leukemia and gastrointestinal stromal tumors, which are characterized by mutations in the Kit gene.
Compound name
Ubiquitin
Catalog number
23-043
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
23-043
Datasheet