VHL complex, active
Complex of N-terminal GST-tagged, recombinant human VHL amino acids 54-end, N-terminal GST tagged, recombinant human Elongin C, untagged, recombinant human Elongin B, N-terminal 6His tagged, recombinant human Cul2 and untagged recombinant human Rbx1 co-expressed by baculovirus in Sf21 insect cells.
Biological information
Background
The VHL complex is a protein complex involved in the regulation of cellular responses to oxygen availability. One of the main functions of the VHL complex is to target the hypoxia-inducible factor (HIF) transcription factors for ubiquitination and subsequent degradation by the proteasome. HIFs are responsible for the expression of genes involved in the response to low oxygen levels, and their degradation by the VHL complex helps to prevent the expression of these genes under normal oxygen conditions. The VHL protein works together with elongin B and elongin C to form a scaffold for the recruitment of the E2 ubiquitin-conjugating enzyme and the E3 ubiquitin ligase complex.
Target class
Ubiquitin
Family
E3
Accession number
GenBank NM_000551 VHL
Target Name
VHL complex, active
Target Alias
HRCA1, RCA1, VHL1, pVHL
Origin
Human
Theori. MW
45 kDa (VHL), 40 kDa (Elongin C), 13 kDa (Elongin B), 91 kDa (Cul2) and 12 kDa (Rbx1)
Affinity tag
N-terminal GST-tag VHL amino, N-Terminal GST tag Elongin C, untagged Elongin B,N-Terminal 6His tag Cul2 and untagged Rbx1
Product specifications
Expression system
expressed in insect cells
Purity
86%
Purification method
Glutathione sepharose
Sample Buffer
0.68 mg/ml of enzyme in 50 mM Tris/HCl pH 7.5, 150 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
The VHL complex is a protein complex involved in the regulation of cellular responses to oxygen availability. One of the main functions of the VHL complex is to target the hypoxia-inducible factor (HIF) transcription factors for ubiquitination and subsequent degradation by the proteasome. HIFs are responsible for the expression of genes involved in the response to low oxygen levels, and their degradation by the VHL complex helps to prevent the expression of these genes under normal oxygen conditions. The VHL protein works together with elongin B and elongin C to form a scaffold for the recruitment of the E2 ubiquitin-conjugating enzyme and the E3 ubiquitin ligase complex.
Compound name
Ubiquitin
Catalog number
23-044
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
23-044
Datasheet