UbcH7, active
N-Terminal HA and 6His-tagged, recombinant, human UbcH7, full length, expressed in E. coli.
Biological information
Background
Ubiquitin-conjugating enzyme E2 L3 (UBE2L3), also known as UbcH7 (Ubiquitin-conjugating enzyme H7), is an enzyme that participates in the process of ubiquitination. UbcH7 is a member of the E2 family of ubiquitin-conjugating enzymes. It plays an important role in the ubiquitination of target proteins, which involves the covalent attachment of ubiquitin molecules to lysine residues on target proteins. UbcH7 is involved in many cellular processes, including DNA repair, cell cycle progression, and regulation of protein stability. Dysregulation of UbcH7 has been associated with the development of certain cancers and other diseases. Therefore, UbcH7 is a potential target for the development of novel therapies for these conditions. Purified using immobilized metal affinity chromatography.
Target class
Ubiquitin
Family
E2
Accession number
GenBank X92962
Target Name
UbcH7, active
Target Alias
E2-F1, L-UBC, UBCH7, UbcM4, UBE2L3
Origin
Human
Theori. MW
20 kDa
Affinity tag
N-terminal HA, 6His-tag
Product specifications
Expression system
expressed in E. coli cells
Purity
90%
Purification method
Immobilized metal affinity chromatography
Sample Buffer
3.26 mg/ml of enzyme in 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
Ubiquitin-conjugating enzyme E2 L3 (UBE2L3), also known as UbcH7 (Ubiquitin-conjugating enzyme H7), is an enzyme that participates in the process of ubiquitination. UbcH7 is a member of the E2 family of ubiquitin-conjugating enzymes. It plays an important role in the ubiquitination of target proteins, which involves the covalent attachment of ubiquitin molecules to lysine residues on target proteins. UbcH7 is involved in many cellular processes, including DNA repair, cell cycle progression, and regulation of protein stability. Dysregulation of UbcH7 has been associated with the development of certain cancers and other diseases. Therefore, UbcH7 is a potential target for the development of novel therapies for these conditions. Purified using immobilized metal affinity chromatography.
Compound name
Ubiquitin
Catalog number
23-047
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
23-047
Datasheet