p38/JTV-1
N-Terminal cMyc and GST-tagged, recombinant, human p38/JTV-1, full length, expressed by baculovirus in Sf21 insect cells.
Biological information
Background
p38 is a member of the mitogen-activated protein kinase (MAPK) family and plays a role in the regulation of cell proliferation, differentiation, and apoptosis. Ubiquitination of p38 has been reported to regulate its stability and activity, with some studies suggesting that it can lead to proteasomal degradation of the protein.
JTV-1 (Jab1/MPN domain-containing metalloenzyme) is a multifunctional protein involved in various cellular processes, including transcriptional regulation, cell cycle control, and DNA damage response. JTV-1 has been shown to interact with and regulate the activity of a number of proteins, including the tumor suppressor p53. Ubiquitination of JTV-1 has been reported to affect its stability and function.
Target class
Ubiquitin
Family
Substrate
Accession number
GenBank BC002853
Target Name
p38/JTV-1
Target Alias
HLD17, JTV-1, JTV1, P38, AIMP2
Origin
Human
Theori. MW
64 kDa
Affinity tag
N-terminal cMyc, GST-tag
Product specifications
Expression system
expressed in insect cells
Purity
75%
Purification method
Glutathione sepharose
Sample Buffer
0.31 mg/ml of enzyme in 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
p38 is a member of the mitogen-activated protein kinase (MAPK) family and plays a role in the regulation of cell proliferation, differentiation, and apoptosis. Ubiquitination of p38 has been reported to regulate its stability and activity, with some studies suggesting that it can lead to proteasomal degradation of the protein.
JTV-1 (Jab1/MPN domain-containing metalloenzyme) is a multifunctional protein involved in various cellular processes, including transcriptional regulation, cell cycle control, and DNA damage response. JTV-1 has been shown to interact with and regulate the activity of a number of proteins, including the tumor suppressor p53. Ubiquitination of JTV-1 has been reported to affect its stability and function.
Compound name
Ubiquitin
Catalog number
23-049
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
23-049
Datasheet