CHIP, active
N-Terminal 6His-tagged, recombinant, human CHIP, full length, expressed in E. coli.
Biological information
Background
CHIP (C-terminus of Hsc70-interacting protein) is a ubiquitin ligase enzyme that plays an important role in regulating protein quality control in cells. As a ubiquitin ligase, CHIP catalyzes the transfer of ubiquitin molecules to specific target proteins, marking them for degradation by the proteasome or for other cellular processes. CHIP is involved in several cellular processes, including protein folding, trafficking, and degradation, as well as DNA damage repair and apoptosis. It interacts with several chaperone proteins, including Hsc70 and Hsp90, and targets misfolded or damaged proteins for ubiquitination and degradation.
The ubiquitination activity of CHIP is regulated by its interaction with several co-chaperones and adapter proteins, including BAG1, BAG2, and HspBP1. These proteins can modulate the substrate specificity and activity of CHIP, as well as its localization within the cell.
Target class
Ubiquitin
Family
E3
Accession number
GenBank NM_005861
Target Name
CHIP, active
Target Alias
CHIP, HSPABP2, NY-CO-7, SCA48, SCAR16, SDCCAG7, UBOX1, STUB1
Origin
Human
Theori. MW
38 kDa
Affinity tag
N-terminal 6His-tag
Product specifications
Expression system
expressed in E. coli cells
Purity
87%
Purification method
Immobilized metal affinity chromatography
Sample Buffer
2.91 mg/ml of enzyme in 50 mM Tris/HCl pH 7.5, 150 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
CHIP (C-terminus of Hsc70-interacting protein) is a ubiquitin ligase enzyme that plays an important role in regulating protein quality control in cells. As a ubiquitin ligase, CHIP catalyzes the transfer of ubiquitin molecules to specific target proteins, marking them for degradation by the proteasome or for other cellular processes. CHIP is involved in several cellular processes, including protein folding, trafficking, and degradation, as well as DNA damage repair and apoptosis. It interacts with several chaperone proteins, including Hsc70 and Hsp90, and targets misfolded or damaged proteins for ubiquitination and degradation.
The ubiquitination activity of CHIP is regulated by its interaction with several co-chaperones and adapter proteins, including BAG1, BAG2, and HspBP1. These proteins can modulate the substrate specificity and activity of CHIP, as well as its localization within the cell.
Compound name
Ubiquitin
Catalog number
23-050
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
23-050
Datasheet