UbcH13/Uev1A, active
Complex of N-Terminal HA and 6His-tagged, recombinant, human UbcH13, full length and untagged, recombinant, human Uev1A, full length, expressed in E. coli.
Biological information
Background
UbcH13 (ubiquitin-conjugating enzyme H13) and Uev1A (ubiquitin-conjugating enzyme E2 variant 1A) are two proteins involved in the process of ubiquitination, a post-translational modification that regulates the activity, stability, and localization of a wide range of cellular proteins. UbcH13 is a member of the E2 family of ubiquitin-conjugating enzymes, which play a key role in transferring ubiquitin molecules to target proteins. Uev1A is an E2 variant, which means it has a slightly different structure compared to other E2 enzymes and can interact with specific E3 ubiquitin ligases.
UbcH13 and Uev1A have been shown to interact with several E3 ligases, including the RING finger protein RNF8, and to be involved in the regulation of DNA damage response and repair. Purified using immobilized metal affinity chromatography.
Target class
Ubiquitin
Family
E2
Accession number
GenBank NM_003348
Target Name
UbcH13/Uev1A, active
Target Alias
UBC13, UbcH13, UBCHBEN, HEL-S-71, UbcH-ben, UBCHBEN, UBC13, CIR1, CROC-1, CROC1, UBE2V, UEV-1, UEV1, UEV1A, UBE2V1
Origin
Human
Theori. MW
19 kDa (UbcH13) and 17 kDa (Uev1A)
Affinity tag
N-terminal HA, 6His-tag UbcH13 and untagged Uev1A
Product specifications
Expression system
expressed in E. coli cells
Purity
99%
Purification method
Immobilized metal affinity chromatography
Sample Buffer
3.08 mg/ml of enzyme in 50 mM Tris/HCl pH 7.5, 150 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
UbcH13 (ubiquitin-conjugating enzyme H13) and Uev1A (ubiquitin-conjugating enzyme E2 variant 1A) are two proteins involved in the process of ubiquitination, a post-translational modification that regulates the activity, stability, and localization of a wide range of cellular proteins. UbcH13 is a member of the E2 family of ubiquitin-conjugating enzymes, which play a key role in transferring ubiquitin molecules to target proteins. Uev1A is an E2 variant, which means it has a slightly different structure compared to other E2 enzymes and can interact with specific E3 ubiquitin ligases.
UbcH13 and Uev1A have been shown to interact with several E3 ligases, including the RING finger protein RNF8, and to be involved in the regulation of DNA damage response and repair. Purified using immobilized metal affinity chromatography.
Compound name
Ubiquitin
Catalog number
23-051
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
23-051
Datasheet