MuRF1, active
N-Terminal GST-tagged, recombinant, human MuRF1, full length, expressed in E. coli.
Biological information
Background
MuRF1 (Muscle RING Finger 1) is a protein that plays a role in the regulation of skeletal muscle mass. It belongs to the RING finger family of E3 ubiquitin ligases. MuRF1 is particularly important in muscle atrophy, the process by which muscle tissue is broken down and lost. During muscle atrophy, MuRF1 is upregulated and targets specific proteins for degradation, including myosin heavy chain, troponin I, and titin.
The ubiquitination of these proteins by MuRF1 leads to their recognition and degradation by the proteasome, which ultimately results in a reduction in muscle mass. Therefore, MuRF1 is a key regulator of muscle homeostasis and is a potential target for therapies aimed at preventing or treating muscle wasting disorders.
Target class
Ubiquitin
Family
E3
Accession number
GenBank NM_032588
Target Name
MuRF1, active
Target Alias
IRF, MURF1, MURF2, RNF28, SMRZ, TRIM63
Origin
Human
Theori. MW
67 kDa
Affinity tag
N-terminal GST-tag
Product specifications
Expression system
expressed in E. coli cells
Purity
80%
Purification method
Glutathione sepharose
Sample Buffer
0.29 mg/ml of enzyme in 50 mM Tris/HCl pH 7.5, 150 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
MuRF1 (Muscle RING Finger 1) is a protein that plays a role in the regulation of skeletal muscle mass. It belongs to the RING finger family of E3 ubiquitin ligases. MuRF1 is particularly important in muscle atrophy, the process by which muscle tissue is broken down and lost. During muscle atrophy, MuRF1 is upregulated and targets specific proteins for degradation, including myosin heavy chain, troponin I, and titin.
The ubiquitination of these proteins by MuRF1 leads to their recognition and degradation by the proteasome, which ultimately results in a reduction in muscle mass. Therefore, MuRF1 is a key regulator of muscle homeostasis and is a potential target for therapies aimed at preventing or treating muscle wasting disorders.
Compound name
Ubiquitin
Catalog number
23-054
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
23-054
Datasheet