XIAP, active
XIAP active. N-terminal GSTtagged, recombinant human XIAP full length, expressed by baculovirus in Sf21 insect cells.
Biological information
Background
X-linked inhibitor of apoptosis (XIAP) is one of a group of intracellular proteins which have been shown to act as endogenous inhibitors of caspases, the enzymatic effectors of apoptosis. XIAP possesses a C-terminal RING domain that functions as an E3 ubiquitin ligase and is able to ubiquitinate a variety of substrates thereby affecting a broad range of cellular activities beyond apoptotic suppression. There is evidence that XIAP plays a significant role in cell division, morphogenesis, heavy metal homeostasis, NF-kB activation and MAP kinase signalling. XIAP expression has been shown to be elevated in several malignancies and so is considered a promising target in anticancer therapies. Expected Purity 89% by SDS-PAGE and Coomassie blue staining.
Target class
Ubiquitin
Family
E3
Accession number
GenBank NM_001167
Target Name
XIAP, active
Target Alias
API3, BIRC4, IAP-3, ILP1, MIHA, XLP2, hIAP-3, hIAP3
Origin
Human
Theori. MW
83 kDa
Affinity tag
N-terminal GST-tag
Product specifications
Expression system
expressed in insect cells
Purity
89%
Purification method
Glutathione sepharose, followed by gel filtration
Sample Buffer
0.17mg/ml of enzyme in 50mM Tris/HCl pH7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
X-linked inhibitor of apoptosis (XIAP) is one of a group of intracellular proteins which have been shown to act as endogenous inhibitors of caspases, the enzymatic effectors of apoptosis. XIAP possesses a C-terminal RING domain that functions as an E3 ubiquitin ligase and is able to ubiquitinate a variety of substrates thereby affecting a broad range of cellular activities beyond apoptotic suppression. There is evidence that XIAP plays a significant role in cell division, morphogenesis, heavy metal homeostasis, NF-kB activation and MAP kinase signalling. XIAP expression has been shown to be elevated in several malignancies and so is considered a promising target in anticancer therapies. Expected Purity 89% by SDS-PAGE and Coomassie blue staining.
Compound name
Ubiquitin
Catalog number
23-056
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
23-056
Datasheet