Smac/DIABLO
C-Terminal cMyc and 6His-tagged, recombinant, human Smac/DIABLO, amino acid residues 56-end, expressed in E. coli.
Biological information
Background
Smac/DIABLO is a protein that plays a critical role in regulating programmed cell death or apoptosis. It is released from the mitochondria into the cytosol in response to various apoptotic stimuli, such as DNA damage or chemotherapeutic agents. By binding to IAPs, Smac/DIABLO enables caspases to cleave their substrates and initiate the cascade of events that ultimately leads to cell death. This mechanism is particularly important in cancer cells, which often have high levels of IAPs that can promote cell survival and resistance to chemotherapy.
The ability of Smac/DIABLO to enhance apoptosis and sensitize cancer cells to chemotherapy has made it a target for the development of cancer therapeutics. Several small-molecule inhibitors of IAPs have been developed that mimic the function of Smac/DIABLO and are being evaluated in clinical trials as potential cancer treatments.
Target class
Ubiquitin
Family
Substrate
Accession number
GenBank NM_019887
Target Name
Smac/DIABLO
Target Alias
DFNA64, SMAC
Origin
Human
Theori. MW
23 kDa
Affinity tag
C-terminal cMyc and 6His-tag
Product specifications
Expression system
expressed in E. coli cells
Purity
94%
Purification method
Immobilized metal affinity chromatography
Sample Buffer
3.012 mg/ml of enzyme in 50 mM Tris/HCl pH 7.5, 150 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
Smac/DIABLO is a protein that plays a critical role in regulating programmed cell death or apoptosis. It is released from the mitochondria into the cytosol in response to various apoptotic stimuli, such as DNA damage or chemotherapeutic agents. By binding to IAPs, Smac/DIABLO enables caspases to cleave their substrates and initiate the cascade of events that ultimately leads to cell death. This mechanism is particularly important in cancer cells, which often have high levels of IAPs that can promote cell survival and resistance to chemotherapy.
The ability of Smac/DIABLO to enhance apoptosis and sensitize cancer cells to chemotherapy has made it a target for the development of cancer therapeutics. Several small-molecule inhibitors of IAPs have been developed that mimic the function of Smac/DIABLO and are being evaluated in clinical trials as potential cancer treatments.
Compound name
Ubiquitin
Catalog number
23-057
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
23-057
Datasheet