Smurf1 active
N-Terminal GST-tagged, recombinant, human Smurf1, full length, expressed in E. coli.
Biological information
Background
SMAD specific E3 ubiquitin protein ligase 1 is an enzyme that belongs to the family of E3 ubiquitin ligases, which are responsible for tagging specific proteins with ubiquitin molecules and targeting them for degradation by the proteasome. It plays a role in several cellular processes, including the regulation of the transforming growth factor-beta (TGF-beta) signaling pathway, which is involved in a variety of biological processes, such as cell growth, differentiation, and apoptosis. SMURF1 has also been implicated in other cellular processes, such as the regulation of cell polarity, cell migration, and the immune response.
The role of SMURF1 in regulating the TGF-beta pathway and its involvement in various cellular processes make it a potential target for the development of therapeutics for several human diseases.
Target class
Ubiquitin
Family
E3
Accession number
GenBank NM_181349
Target Name
Smurf1 active
Target Alias
Origin
Human
Theori. MW
110 kDa
Affinity tag
N-terminal GST-tag
Product specifications
Expression system
expressed in E. coli
Purity
59%
Purification method
Glutathione sepharose
Sample Buffer
0.19 mg/ml of enzyme in 50 mM Tris/HCl pH 7.5, 150 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
SMAD specific E3 ubiquitin protein ligase 1 is an enzyme that belongs to the family of E3 ubiquitin ligases, which are responsible for tagging specific proteins with ubiquitin molecules and targeting them for degradation by the proteasome. It plays a role in several cellular processes, including the regulation of the transforming growth factor-beta (TGF-beta) signaling pathway, which is involved in a variety of biological processes, such as cell growth, differentiation, and apoptosis. SMURF1 has also been implicated in other cellular processes, such as the regulation of cell polarity, cell migration, and the immune response.
The role of SMURF1 in regulating the TGF-beta pathway and its involvement in various cellular processes make it a potential target for the development of therapeutics for several human diseases.
Compound name
Ubiquitin
Catalog number
23-058
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
23-058
Datasheet