TRAF6, active
N-Terminal GST-tagged, recombinant, human TRAF6, full length, expressed in E. coli.
Biological information
Background
TRAF6 is a protein that is involved in a variety of cellular processes, including the regulation of the immune response, inflammation, and bone metabolism. TRAF6 functions as an E3 ubiquitin ligase. One of the key functions of TRAF6 is its role in the activation of NF-kappaB, a transcription factor that regulates the expression of genes involved in the immune response and inflammation. Upon activation of cell surface receptors such as Toll-like receptors (TLRs) or interleukin-1 receptors (IL-1Rs), TRAF6 is recruited to the receptor complex and promotes the activation of NF-kappaB through the ubiquitination of downstream signaling molecules.
TRAF6 has also been implicated in the regulation of bone metabolism, specifically in the differentiation and activation of osteoclasts, which are the cells responsible for bone resorption.
Target class
Ubiquitin
Family
E3
Accession number
GenBank BC031052
Target Name
TRAF6, active
Target Alias
MGC:3310, RNF85
Origin
Human
Theori. MW
87 kDa
Affinity tag
N-terminal GST-tag
Product specifications
Expression system
expressed in E. coli cells
Purity
54%
Purification method
Glutathione sepharose
Sample Buffer
0.65 mg/ml of enzyme in 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
TRAF6 is a protein that is involved in a variety of cellular processes, including the regulation of the immune response, inflammation, and bone metabolism. TRAF6 functions as an E3 ubiquitin ligase. One of the key functions of TRAF6 is its role in the activation of NF-kappaB, a transcription factor that regulates the expression of genes involved in the immune response and inflammation. Upon activation of cell surface receptors such as Toll-like receptors (TLRs) or interleukin-1 receptors (IL-1Rs), TRAF6 is recruited to the receptor complex and promotes the activation of NF-kappaB through the ubiquitination of downstream signaling molecules.
TRAF6 has also been implicated in the regulation of bone metabolism, specifically in the differentiation and activation of osteoclasts, which are the cells responsible for bone resorption.
Compound name
Ubiquitin
Catalog number
23-059
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
23-059
Datasheet