c-Cbl (c-Myc tagged) activated
N-Terminal c-Myc and GST-tagged, recombinant, human c-Cbl, full length, expressed by baculovirus in Sf21 insect cells.
Biological information
Background
c-Cbl is a cellular protein that belongs to the family of Cbl proteins. It acts as an E3 ubiquitin ligase and is involved in regulating the degradation of various proteins in the cell. c-Cbl has several functions in different cellular processes, including receptor downregulation, signal transduction, and cytoskeletal organization. c-Cbl is also involved in signal transduction, where it can be recruited to activated receptors or intracellular signaling proteins to regulate their downstream signaling pathways.
Dysregulation of c-Cbl has been associated with several human diseases, including cancer, immune disorders, and neurodegenerative diseases. Purified using glutathione sepharose, activated in vitro with Src (cat # 14-746).
Target class
Ubiquitin
Family
E3
Accession number
GenBank NM_005188
Target Name
c-Cbl (c-Myc tagged) activated
Target Alias
C-CBL, CBL2, FRA11B, NSLL, RNF55
Origin
Human
Theori. MW
129 kDa
Affinity tag
N-terminal c-Myc, GST-tag
Product specifications
Expression system
expressed in insect cells
Purity
66%
Purification method
Glutathione sepharose
Sample Buffer
0.629 mg/ml of enzyme in 30 mM Tris/HCl pH 7.5, 10 mM MOPS pH 7.5, 90 mM NaCl, 12 mM MgCl2, 0.05 µM Src, 100 µM ATP, 0.06 mM EGTA, 0.018% Brij-35, 0.004% Tween 20, 5 mM β-glycerophosphate, 1 mM sodium orthovanadate, 162 mM sucrose, 0.6 mM benzamidine, 0.12 mM PMSF, 0.06% 2-mercaptoethanol, Frozen solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
c-Cbl is a cellular protein that belongs to the family of Cbl proteins. It acts as an E3 ubiquitin ligase and is involved in regulating the degradation of various proteins in the cell. c-Cbl has several functions in different cellular processes, including receptor downregulation, signal transduction, and cytoskeletal organization. c-Cbl is also involved in signal transduction, where it can be recruited to activated receptors or intracellular signaling proteins to regulate their downstream signaling pathways.
Dysregulation of c-Cbl has been associated with several human diseases, including cancer, immune disorders, and neurodegenerative diseases. Purified using glutathione sepharose, activated in vitro with Src (cat # 14-746).
Compound name
Ubiquitin
Catalog number
23-061
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
23-061
Datasheet