Idol (c-Myc tagged), active
N-Terminal c-Myc and 6His-tagged, recombinant, human Idol, full length, expressed by baculovirus in Sf21 insect cells.
Biological information
Background
Idol is a protein that plays a role in the regulation of cholesterol metabolism in the body. It acts as an E3 ubiquitin ligase and is responsible for the degradation of the low-density lipoprotein receptor (LDLR), which is important for the uptake of cholesterol by cells.
Idol is induced by the liver X receptor (LXR), which is a nuclear receptor that regulates cholesterol homeostasis. When cholesterol levels are high, LXR induces the expression of Idol, which leads to the ubiquitination and degradation of the LDLR in the cell. This process reduces the uptake of cholesterol by the cell and prevents the accumulation of excess cholesterol. Dysregulation of Idol has been associated with various human diseases, including atherosclerosis and cardiovascular disease. Therefore, Idol is a potential therapeutic target for the treatment of these diseases.
Target class
Ubiquitin
Family
E3
Accession number
GenBank BC002860
Target Name
Idol (c-Myc tagged), active
Target Alias
IDOL, MIR, MYLIP
Origin
Human
Theori. MW
55 kDa
Affinity tag
N-terminal c-Myc, 6His-tag
Product specifications
Expression system
expressed in insect cells
Purity
83%
Purification method
Immobilized metal affinity chromatography
Sample Buffer
0.559 mg/ml of enzyme in 50 mM Tris/HCl pH 7.5, 150 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
Idol is a protein that plays a role in the regulation of cholesterol metabolism in the body. It acts as an E3 ubiquitin ligase and is responsible for the degradation of the low-density lipoprotein receptor (LDLR), which is important for the uptake of cholesterol by cells.
Idol is induced by the liver X receptor (LXR), which is a nuclear receptor that regulates cholesterol homeostasis. When cholesterol levels are high, LXR induces the expression of Idol, which leads to the ubiquitination and degradation of the LDLR in the cell. This process reduces the uptake of cholesterol by the cell and prevents the accumulation of excess cholesterol. Dysregulation of Idol has been associated with various human diseases, including atherosclerosis and cardiovascular disease. Therefore, Idol is a potential therapeutic target for the treatment of these diseases.
Compound name
Ubiquitin
Catalog number
23-064
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
23-064
Datasheet