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STEAP2, Active Protein

Six Transmembrane Epithelial Antigen of the Prostate 2

Biological information

Background

STEAP2 is a member of the STEAP (Six Transmembrane Epithelial Antigen of the Prostate) hemoproteins exhibiting metal ion reductase activities. STEAP2 has a high level of expression in protaste epithelial cells and is located at the plasma membrane, golgi complex, trans golgi network (TGN) and early endosomes. STEAP2 works as a shuttle between Golgi and plasma membrane, contributing to Fe3+ and Cu2+ uptake and reduction, facilitating transferrin cycle. Moreover, its association with early endosomes and the TGN suggest its implication in protein delivery and sorting mechansims. STEAP2 is highly over expressed in prostate cancer cells, promoting tumors proliferation by modulating aggressive prostate cancer traits such as cell migration and invasion. Even if STEAP2 shows promise as a potential novel therapeutic target, no treatment has been developed to date.

Target class
Enzyme
Family
Transmembrane receptor
Accession number
Q8NFT2
Target Name
STEAP2, Active Protein
Target Alias
Six Transmembrane Epithelial Antigen of the Prostate 2
Origin
Human
Theori. MW
57 kDa
Affinity tag
HRV3C/muGFP/Flag/TwinStrep

Product specifications

Expression system
Expression in HEK 293F
Purity
Refer to CoA for Purity
Purification method
StrepTactin, muGFP HRV3C cleavage and SEC purification
Sample Buffer
25 mM NaH2PO4, 150mM NaCl, 10% glycerol, 0.005 % - 0.0005% LMNG-DCOD
Specified activity
Refer to CoA
Application
Hit finding, SBDD, Small molecules screening
Storage conditions
Store at -80°C, avoid freeze-thaw cycle
Usage disclaimer
Hit finding, SBDD, Small molecules screening

Chemical data

Background

STEAP2 is a member of the STEAP (Six Transmembrane Epithelial Antigen of the Prostate) hemoproteins exhibiting metal ion reductase activities. STEAP2 has a high level of expression in protaste epithelial cells and is located at the plasma membrane, golgi complex, trans golgi network (TGN) and early endosomes. STEAP2 works as a shuttle between Golgi and plasma membrane, contributing to Fe3+ and Cu2+ uptake and reduction, facilitating transferrin cycle. Moreover, its association with early endosomes and the TGN suggest its implication in protein delivery and sorting mechansims. STEAP2 is highly over expressed in prostate cancer cells, promoting tumors proliferation by modulating aggressive prostate cancer traits such as cell migration and invasion. Even if STEAP2 shows promise as a potential novel therapeutic target, no treatment has been developed to date.

Compound name
Enzyme
Catalog number
41-O101A-10UG
Molecular formula
CAS
MW
Ka
Percent composition

Product specifications

Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
Hit finding, SBDD, Small molecules screening
Solubility structure
Datasheets
41-O101A
Datasheet
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