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CD20

Recombinant Human Cluster of Differentiation 20

Biological information

Background

CD20 is a transmembrane receptor, specific of normal and malignant B-cells. Indeed, CD20 is involved in development and differentiation of B-cells. It boosts calcium signal indirectly and regulates their cellular signalization. CD20 can be subjected by post-translational modifications (phosphorylation). Moreover, it can be found as a double-barrel dimer. As of today, there is no natural ligand known and its function is still debated. CD20 transmembrane receptor is targeted in various diseases like cancer (non-Hodgkin’s lymphoma and Chronic lymphocytic leukemia’s), multiple sclerosis, or autoimmune disorders. The treatment most used for lymphoid malignancies is the Rituximab, a monoclonal antibody, which induced the clustering of CD20 in lipid raft. Thus, this triggers several immune defense mechanisms, including antibody-dependent cell-mediated cytotoxicity (ADCC), complement-dependent cytotoxicity (CDC) and antibody-dependent cellular phagocytosis (ADCP).

Target class
Receptor
Family
Transmembrane receptor
Accession number
P11836
Target Name
CD20
Target Alias
B-lymphocyte surface antigen B1, Bp35, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1
Origin
Human
Theori. MW
40.8 kDa
Affinity tag
Avi/TwinStrep/His (both C-terminal)

Product specifications

Expression system
Expression in HEK 293F
Purity
Refer to CoA for Purity
Purification method
StrepTactin purification
Sample Buffer
Protein in 50 mM Tris, 150mM NaCl, 10% glycerol, 0.03%-0.003% DDM-CHS
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
Store at -80°C, avoid freeze-thaw cycle
Usage disclaimer
For Research Only

Chemical data

Background

CD20 is a transmembrane receptor, specific of normal and malignant B-cells. Indeed, CD20 is involved in development and differentiation of B-cells. It boosts calcium signal indirectly and regulates their cellular signalization. CD20 can be subjected by post-translational modifications (phosphorylation). Moreover, it can be found as a double-barrel dimer. As of today, there is no natural ligand known and its function is still debated. CD20 transmembrane receptor is targeted in various diseases like cancer (non-Hodgkin’s lymphoma and Chronic lymphocytic leukemia’s), multiple sclerosis, or autoimmune disorders. The treatment most used for lymphoid malignancies is the Rituximab, a monoclonal antibody, which induced the clustering of CD20 in lipid raft. Thus, this triggers several immune defense mechanisms, including antibody-dependent cell-mediated cytotoxicity (ADCC), complement-dependent cytotoxicity (CDC) and antibody-dependent cellular phagocytosis (ADCP).

Compound name
Receptor
Catalog number
41-R501A-10UG
Molecular formula
CAS
MW
Ka
Percent composition

Product specifications

Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
41-R501A
Datasheet
€ 490,00 EUR
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