hPHF8
N-terminal 6His-tagged recombinant amino acids 79-447, human PHF8.
Biological information
Background
hPHF8 (plant homeodomain finger protein 8) is a histone demethylase that belongs to the PHF (plant homeodomain finger) family. hPHF8 specifically targets tri-methylated lysine 9 and 27 on histone H3 (H3K9me3 and H3K27me3, respectively), and catalyzes their removal, leading to changes in chromatin structure and gene expression. hPHF8 has been implicated in the regulation of several diseases, including cancer, intellectual disability, and cleft lip and palate. Mutations in the PHF8 gene have been associated with cases of X-linked mental retardation and various developmental disorders. hPHF8 has also been shown to play a role in the regulation of androgen receptor signaling, which is involved in the development and progression of prostate cancer. hPHF8 is an important epigenetic protein with potential therapeutic applications. Its inhibition has been shown to suppress tumor growth and improve response to chemotherapy in prostate cancer.
Target class
Epigenetic
Family
Accession number
NP_055922.1
Target Name
hPHF8
Target Alias
DKFZp686E0868, JHDM1F, KIAA1111, MRXSSD, ZNF422
Origin
Human
Theori. MW
56.8 kDa
Affinity tag
6His
Product specifications
Expression system
Expressed in E. coli
Purity
Refer to CoA for Purity
Purification method
Immobilised metal affinity chromatography
Sample Buffer
Protein in 25mM
Hepes/NaOH pH 7.5, 250mM NaCl, 50% glycerol.
Frozen solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C.
Usage disclaimer
For Research Only
Chemical data
Background
hPHF8 (plant homeodomain finger protein 8) is a histone demethylase that belongs to the PHF (plant homeodomain finger) family. hPHF8 specifically targets tri-methylated lysine 9 and 27 on histone H3 (H3K9me3 and H3K27me3, respectively), and catalyzes their removal, leading to changes in chromatin structure and gene expression. hPHF8 has been implicated in the regulation of several diseases, including cancer, intellectual disability, and cleft lip and palate. Mutations in the PHF8 gene have been associated with cases of X-linked mental retardation and various developmental disorders. hPHF8 has also been shown to play a role in the regulation of androgen receptor signaling, which is involved in the development and progression of prostate cancer. hPHF8 is an important epigenetic protein with potential therapeutic applications. Its inhibition has been shown to suppress tumor growth and improve response to chemotherapy in prostate cancer.
Compound name
Epigenetic
Catalog number
EPI049
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
EPI049
Datasheet