SETD8
Recombinant human SETD8 amino acids 186-352.
Biological information
Background
SETD8 (SET domain-containing protein 8), also known as PR-Set7, is a histone lysine methyltransferase that catalyzes the transfer of a methyl group from S-adenosylmethionine (SAM) to the lysine 20 residue of histone H4 (H4K20), leading to the formation of monomethylated H4K20. SETD8 is a member of the SET domain-containing protein family and is the only known enzyme that specifically methylates H4K20 in a monomethylated form. SETD8 plays a crucial role in the regulation of DNA replication by methylating H4K20 on newly synthesized chromatin, leading to the recruitment of checkpoint proteins and the maintenance of genomic stability. It also plays a role in the DNA damage response by promoting the recruitment of repair factors to damaged DNA. SETD8 is an important epigenetic protein with potential therapeutic applications. Its inhibition has been shown to suppress tumor growth and improve the response to chemotherapy in preclinical models of cancer.
Target class
Epigenetic
Family
Accession number
NP_065115.3
Target Name
SETD8
Target Alias
Origin
Human
Theori. MW
19.2 kDa
Affinity tag
Thrombin protease
Product specifications
Expression system
Expressed in E. coli
Purity
Refer to CoA for Purity
Purification method
Immobilised metal affinity chromatography
Sample Buffer
Protein in 25mM Tris/HCl pH 7.5, 250mM NaCl, 50% glycerol. Frozen solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C.
Usage disclaimer
For Research Only
Chemical data
Background
SETD8 (SET domain-containing protein 8), also known as PR-Set7, is a histone lysine methyltransferase that catalyzes the transfer of a methyl group from S-adenosylmethionine (SAM) to the lysine 20 residue of histone H4 (H4K20), leading to the formation of monomethylated H4K20. SETD8 is a member of the SET domain-containing protein family and is the only known enzyme that specifically methylates H4K20 in a monomethylated form. SETD8 plays a crucial role in the regulation of DNA replication by methylating H4K20 on newly synthesized chromatin, leading to the recruitment of checkpoint proteins and the maintenance of genomic stability. It also plays a role in the DNA damage response by promoting the recruitment of repair factors to damaged DNA. SETD8 is an important epigenetic protein with potential therapeutic applications. Its inhibition has been shown to suppress tumor growth and improve the response to chemotherapy in preclinical models of cancer.
Compound name
Epigenetic
Catalog number
EPI057
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
EPI057
Datasheet