Purified Membrane Proteins

Multidrug resistance ABC transporter ATP-binding/permease protein BmrA - Transporter

CALIXAR’s BmrA membrane protein (Multidrug resistance ABC transporter ATP-binding/permease protein BmrA) is an efflux protein and ABC transporter that acts as a mechanical pump for ATP dependent drug efflux. This unique characteristic makes BmrA membrane protein a powerful therapeutic target for multi-drug resistance studies.

Multidrug resistance ABC transporter ATP-binding/permease protein BmrA is a powerful therapeutic target for multi-drug resistance studies, ideal for therapeutic indications such as bacterial resistance to antibiotics.

Purchase Multidrug resistance ABC transporter ATP-binding/permease protein BmrA

CALIXAR’s BmrA membrane targets aid in the exploration, improvement, validation of novel molecules and therapeutic antibodies. Our Multidrug resistance ABC transporter ATP-binding/permease protein BmrA  is excellent for therapeutic indications such as bacterial resistance to antibiotics.

Target name: Multidrug resistance ABC transporter ATP-binding/permease protein BmrA

Gene: bmrA

Uniprot Accession: O06967

Origin: Bacillus subtilis (strain 168)

Class: Multidrug resistance ABC transporter

Sequence: Full-length, wildtype sequence, with a N-terminus 6xHis-tag.

Affinity Tag​: His-tag (N-terminal)

Catalogue number: PP3

Theor. MW: 64.5 kDa​

Shipment temperature: Dry Ice

Storage conditions: Store at -80°C​

Expression system: Escherichia coli (BL21C43)

Purity: >90%

Purification: Immobilized Metal Affinity Chromatography

Activity: Confirmed by ATPase activity assay

Concentration​: Up to 5mg/ml

Sample buffer: 50mM Tris-Cl pH 8.0, 100 mM NaCl, 0.01% DDM, 10%glycerol

Available quantity: From 10µg up to mg scale

€200.00 (10µg)

Ships in 7 business days

First in market

CALIXAR is the first to produce native, wild-type, unmutated and untruncated targets.

Better discovery

Develop reliable and effective drugs and antibodies as they are not locked in any specific conformation.

Why choose us?

Multidrug resistance ABC transporter ATP-binding/permease protein BmrA: We utilize a unique & custom method to give us the edge

CALIXAR’s Multidrug resistance ABC transporter ATP-binding/permease protein BmrA  expedites reliable fragment-based drug design (FBDD), structure-based medication discovery (SBDD) and antibody development against this specific target.

Unlike CALIXAR’s BmrA membrane targets, other alternative approaches result in a BmrA membrane protein that becomes mutated and truncated.

As with all transporters, Multidrug resistance ABC transporter ATP-binding/permease protein BmrA is unpredictable targets, difficult to produce natively without particular technology. Traditionally, BmrA membrane proteins were often developed in a solution that naturally could not be pure, nor native (truncated, mutated), and consequently are moderately unstable.

Thanks to CALIXAR’s expertise, we are able to deliver BmrA membrane targets that maintain the structure and function of the target. Our Multidrug resistance ABC transporter ATP-binding/permease protein BmrA is pure, native, unaltered and stable.

CALIXAR has the capacity to provide high quality, native, wild-type, unaltered, unmutated and untruncated targets for antibody development and pharmaceutical discovery projects. We are unparalleled in the market and at the vanguard of Membrane Protein technology.

CALIXAR’s Multidrug resistance ABC transporter ATP-binding/permease protein BmrA is the first native full-length and functional target on the market. Other existing BmrA membrane targets are either mutated or truncated. Our BmrA membrane protein is produced in a prokaryotic system.

CALIXAR’s BmrA membrane target is high-quality membrane proteins utilized for (bio)drug discovery and is adapted for use in pharmaceutical companies, biotechnology companies, as well as for academic teams that are involved in the life science domains.

  • Antibodies (including nanobodies, scaffold proteins, aptamers)
  • Small molecules
  • 3D Structures (classical X-ray or XFEL, Cryo-EM, NMR)
  • Drug discovery (Screening: HTS, FBDD, SBDD; Hit and lead validation)
  • Antibody discovery (Immunization and display technologies)
  • Clinical stage (drug validation on reliable native BmrA)​

Multidrug resistance ABC transporter ATP-binding/permease protein BmrA references

ANALYTICAL BIOCHEMISTRY

Novel systematic detergent

Desuzinges Mandon E. et al. 2017 

PLoS One

Structuring detergents for extracting and stabilizing functional membrane proteins.

Matar-Merheb R. et al. 2011 

 

Biochem J.

The ABC transporter BmrA from Bacillus subtilis is a functional dimer when in a detergent-solubilized state.

Stéphanie Ravaud et al. 2017 

Membrane Protein Purchase

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